摘要 |
This invention provides a method for simultaneously detecting the presence of at least two enzymes in a sample, said method comprising the steps of; i) providing a first substrate for a first enzyme, said first substrate being labeled with a first fluorophore, ii) providing a second substrate for a second enzyme, said second substrate being labeled with a second fluorophore, iii) exposing the labeled substrates to the sample to allow the first and second enzymes present in the sample to interact with respective first and second fluorophore-labeled substrates to form respective first and second fluorophore-labeled substrate fragments; and, detecting the presence of said fluorophore-labeled substrate fragments.
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