MICROFLUIDICDEVICE FOR TRAPPING SINGLE CELL

摘要

It is intended to provide a microfluidicdevice whereby a large amount cells in a sample can be highly efficiently isolated and trapped at the single cell level without damaging the cells by using a microfabrication technique. A microfluidicdevice which comprises, in the following order: a top substrate made of a plastic plate having a channel for forming a microchannel connecting a sample inlet to a sample outlet; an upper plastic plate constituting the microchannel together with the above-described top substrate and having an aperture for a micromesh; a holder plate holding a plastic micromesh having a plural number of micro-cavities for trapping cells; a lower plastic plate having an aperture for suction; and a bottom substrate made of a plastic plate having a groove for forming a suction channel, wherein the micro-cavities for capturing cells in the above-described micromesh have an inverted pot shape or a cylindrical shape and the above-described upper plate is made of a plastic material that is softer than the plastic material for the above-described top substrate.