| 摘要 |
The present invention provides a novel Kunitz-type inhibitor protein exhibiting high potency to inhibit serine protease activity of human blood coagulation factor Xa. The Kunitz-type inhibitor protein against human blood coagulation factor Xa according to the present invention is a Kunitz-type inhibitor protein: bitoran D from Bitis arietans venom having the following amino-acid sequence I: <tables id="TABLE-US-00001" num="00001"> <table frame="none" colsep="0" rowsep="0" tabstyle="monospace"> <tgroup align="left" colsep="0" rowsep="0" cols="2"> <colspec colname="1" colwidth="217pt" align="right"/> <colspec colname="2" colwidth="0pt" align="left"/> <tbody valign="top"> <row> <entry>(SEQ ID NO:1)</entry> <entry/> </row> </tbody> </tgroup> <tgroup align="left" colsep="0" rowsep="0" cols="2"> <colspec colname="1" colwidth="217pt" align="left"/> <colspec colname="2" colwidth="0pt" align="left"/> <tbody valign="top"> <row> <entry>SKKRP DFCYL PADDG PCRAF IPSFY YNSTS NECNT FIYGG</entry> <entry/> </row> <row> <entry> </entry> </row> <row> <entry>CYGNA NKFES MDECR KTCVA SA,</entry> </row> </tbody> </tgroup> </table> </tables> a Kunitz-type inhibitor protein: bitoran V from Bitis arietans venom having the following amino-acid sequence II: <tables id="TABLE-US-00002" num="00002"> <table frame="none" colsep="0" rowsep="0" tabstyle="monospace"> <tgroup align="left" colsep="0" rowsep="0" cols="2"> <colspec colname="1" colwidth="217pt" align="right"/> <colspec colname="2" colwidth="0pt" align="left"/> <tbody valign="top"> <row> <entry>(SEQ ID NO:2)</entry> <entry/> </row> </tbody> </tgroup> <tgroup align="left" colsep="0" rowsep="0" cols="2"> <colspec colname="1" colwidth="217pt" align="left"/> <colspec colname="2" colwidth="0pt" align="left"/> <tbody valign="top"> <row> <entry>C RQNRP DFCYL PAVEG PCRAY IRSFF YNSTS NECEK FFYGG</entry> <entry/> </row> <row> <entry> </entry> </row> <row> <entry>CYGNA NKFET RDECR KTCVA SA,</entry> </row> </tbody> </tgroup> </table> </tables> or a modified protein thereof.
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