Determination of the activity of proteases

摘要

A method for determining the activity of proteases is provided. Fluorogenic substrates from which the fluorogen 7-amino-4-trifluoromethylcoumarin is eliminated proved to be particularly advantageous for the activity measurement. These substrates make it possible for measurement in microtiter plates with a fluorescence reader and thus for the fluorimetric determination of such enzyme activities in blood serum.