发明名称 |
Method for enhancing keratinocyte stem cells |
摘要 |
The enrichment of a population of keratinocyte stem cells (KSCs) from a preparation of keratinocytes and KSCs, includes pre-enrichment by contacting a sample of keratinocytes and KSCs with a collagen-coated culture plate for a sufficient time for KSCs to adhere, followed by washing away the non-adherent cells and recovering the adherent cells. The recovered adherent cells are sorted by their expression level of EGFR so that cells are recovered that have an expression level of EGFR of less than about 50% of the maximum level of EGFR expression, and the recovered cells have an expansion potential of at least 109 after about 100 days in culture.
|
申请公布号 |
US7476538(B2) |
申请公布日期 |
2009.01.13 |
申请号 |
US20040494275 |
申请日期 |
2004.12.15 |
申请人 |
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE;L'OREAL |
发明人 |
HATZFELD JACQUES;FORTUNEL NICOLAS;HATZFELD ANTOINETTE |
分类号 |
C12N1/00;C12N5/00;A61K35/12;A61L27/00;C12N5/071;C12N5/074 |
主分类号 |
C12N1/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|