| 摘要 |
<p>The present invention relates to a novel method for rejuvenating isolated pancreatic islets for transplantation, more particularly to a method to increase or enhance insulin content, glucose-stimulated insulin secretion, viability, and engraft capacity upon transplantation into diabetic recipients of isolated pancreatic islets. According the present invention, the rejuvenated islets have 1.7-fold and 1.9-fold greater intracellular insulin content and glucose-stimulated insulin secretion (GSIS), respectively than freshly isolated islets. Moreover, many genes important for ß-cell survival and function such as insulin, GLUT2, Bcl-2, and DNA repair enzymes were significantly increased by rejuvenation. These enhanced islet capacity endow them greater resistance against oxidative stresses, reduced immunogenicity, and at least 2-fold increase of engraft efficacy in islet xeno- and allogeneic transplantation model. In conclusion, according to the present invention, it is possible that islet transplantation with smaller amount of islets should cure type 1 diabetes.</p> |
