摘要 |
<p>PURPOSE:To carry out the culture of cells in a culture tank containing a draft tube or a built-in straightening vane in high efficiency by adjusting the gap between the lower end of the draft tube or the straightening vane and the bottom of the tank in such a manner as to get the cell concentration difference between the bottom and the inside of the tank to be fallen within a prescribed range. CONSTITUTION:Living cells are cultured in a liquid in suspended state under aeration using a culture tank containing a draft tube or a built-in straightening vane. In the above process, the gap between the lower end of the draft tube or the straightening vane and the bottom of the tank is adjusted in such a manner as to get the cell concentration difference between the bottom and the inside of the tank to be fallen within a prescribed range. The cells are smoothly fluidized and cultured without causing the precipitation of the cells even under low rate of aeration.</p> |