| 摘要 |
The invention provides an automated VNTR genotyping method using multiple variable-number tandem repeats (VNTRs) loci based on mycobacterial interspersed repetitive units (MIRU) undergoing multiplex PGR and high throughput MEGABACE(R) capillary electrophoresis system. The method uses fluorescent dyes of 6-carboxytetramethylrhodamine(TAMRA), 6-carboxy fluorescein (FAM) and 6-carboxy-2', 4, 4', 5', 7, 7'-hexachlorofluorescein (HEX) labeling PGR primers. The method results in an efficient VNTR genotyping with low cost, less labor-requirement and less reaction time. The method is applicable in organism analyses by VNTR genotyping, such as microorganisms, parasites, animals or plants.
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