| 摘要 |
<p>SUSHI PEPTIDE MULTIMER Endotoxin, also known as lipopolysaccharides (LPS), is the major mediator of septic shock due to Gram-negative bacterial infection. Chemically synthesized S3 peptide, derived from Sushi3 domain of Factor C, which is the endotoxin- sensitive serine protease of the limulus coagulation cascade, binds and neutralizes LPS activity. Fluorescent tagged-S3 is shown to detect LPS-containing bacteria. For large-scale production of S3 and to mimick other pathogen- recognizing molecules, tandem multimers of the S3 gene were constructed and expressed in E. coll. Tetramer of S3 for example is shown to display an enhanced inhibitory effect on LPS- induced activities. An affinity matrix based on tetramer of S3 is also shown to be particularly efficient at removing LPS.</p> |
