发明名称 |
Methods for antibody engineering |
摘要 |
The invention provides a method for identifying positions of an antibody that can be modified without significantly reducing the binding activity of the antibody. In many embodiments, the method involves identifying a substitutable position in a parent antibody by comparing its amino acid sequence to the amino acid sequences of a number of related antibodies that each bind to the same antigen as the parent antibody. The amino acid at the substitutable position may be substituted for a different amino acid without significantly affecting the activity of the antibody. The subject methods may be employed to change the amino acid sequence of a CDR without significantly reducing the affinity of the antibody of the antibody, in humanization methods, or in other antibody engineering methods. The invention finds use in a variety of therapeutic, diagnostic and research applications.
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申请公布号 |
US2006099204(A1) |
申请公布日期 |
2006.05.11 |
申请号 |
US20040984473 |
申请日期 |
2004.11.08 |
申请人 |
COUTO FERNANDO JOSE R D;HENDRICKS KRISTIN B;WALLACE S E;YU GUO-LIANG |
发明人 |
COUTO FERNANDO JOSE R.D.;HENDRICKS KRISTIN B.;WALLACE S. E.;YU GUO-LIANG |
分类号 |
G01N33/53;A61K39/395;C07K16/18;C12N5/06;C12P21/06;G06F19/00 |
主分类号 |
G01N33/53 |
代理机构 |
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地址 |
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