| 摘要 |
The present invention provides a method and an assay kit for detecting ubiquitin ligase (E3) activity in a sample. The method includes the steps of (1) preparing a sample mixture comprising ubiquitin, a ubiquitin activating enzyme (E1), a ubiquitin conjugating enzyme (E2), a reaction buffer, and the sample; (2) incubating the sample mixture under conditions suitable for ubiquitination to occur; and (3) detecting a presence of ubiquitination products in the sample mixture after the incubation.
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