PHYTASE-CONTAINING AQUEOUS LIQUID, METHOD FOR PRODUCTION THEREOF, PHYTASE-CONTAINING GRANULATED MATERIAL AND METHOD FOR PRODUCTION THEREOF, ANIMAL FEED, PREMIX OR SEMI-FINISHED ANIMAL FEED, METHOD FOR PRODUCTION THEREOF, AND ANIMAL GROWTH STIMULATION

摘要

FIELD: biotechnology, feedstuff production. ^ SUBSTANCE: method for phytase-containing aqueous liquid includes cultivation of genus Aspergillus microorganism transformed with expression vector, containing phytase gene of said microorganism bond to promoter and/or signal sequence of amyloglucosidase gene. Cultivation is carried out in aqueous medium containing carbon and nitrogen source under conditions, which allow recombinant phytase expression. Cultural liquid is filtered, cation exchange chromatography, anion exchange chromatography, and ultrafiltration are carried out to produce phytase-containing aqueous liquid having activity at least of 14000 U/g. Said aqueous liquid with phytase activity is useful in production of granulated material, animal feed, premix or semi-finished animal feed. ^ EFFECT: granulated material and animal feed for animal growth stimulation. ^ 24 cl, 2 tbl, 10 ex