NEUROTRANSMITTER SENSORS AND METHODS OF USING THE SAME

摘要

Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotranmitter concentrations in vivo and in culture.