发明名称 |
SYSTEMS AND METHODS FOR DETECTING NUCLEIC ACIDS |
摘要 |
<p>A method and kit for detecting a target nucleic acid in a sample is described. The sample to be analyzed may include a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising exonuclease activity that can cleave the hybridized hybridization probe to thereby generate a labeled probe fragment. At least one portion of the hybridization probe hybridizes to another portion of the hybridization probe to thereby form a folded structure. The method can involve melting the sample, reducing the temperature of the sample to allow primer and probe to each hybridize to at least a portion of single stranded target nucleic acid in the sample, elongating the primer and releasing the labeled probe fragment. The sample can be contacted with a solid support comprising surface bound capture probes which hybridize to the labeled probe fragments. The label can then be detected.</p> |
申请公布号 |
WO2008083259(A1) |
申请公布日期 |
2008.07.10 |
申请号 |
WO2007US89007 |
申请日期 |
2007.12.28 |
申请人 |
APPLERA CORPORATION;AIVAZACHVILI, VISSARION;SCABOO, KRISTIAN;SPIER, EUGENE |
发明人 |
AIVAZACHVILI, VISSARION;SCABOO, KRISTIAN;SPIER, EUGENE |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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