| 摘要 |
A gene detection method comprises an immobilization step of forming a single-stranded capture probe having a base sequence that is complementary to the target gene to be detected, and immobilizing the capture probe to a solid phase; a gene sample formation step of forming a gene sample by denaturing the target gene into a single strand; a bonding step of adding an electrochemically active substance to the gene sample to chemical bond's the gene sample with the electrochemically active substance; a gene sample capturing process of hybridizing the gene sample to which the electrochemically active substance is bonded, with the single-stranded capture probe that is immobilized to the solid phase, thereby to make the solid phase capture the gene sample; and a detection step of detecting the electrochemically active substance that is bonded to the gene sample immobilized to the solid phase, by electrochemical measurement.
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