摘要 |
A method for obtaining an entomopathogenous viral insecticide includes selecting and using a producent strain and also filler substances. Collecting caterpillars of the v-looper (Itame wauaria Z.) and common magpie moth (Abraxas grossularia Z.) is carried out from currant and gooseberry plantations, they are kept without feed in the laboratory conditions. The caterpillars are fed with black currant leaves, preliminary infected with an original strain of the nuclear polyhedrosis virus. After death of caterpillars they are cut, ground in a mortar, water is added, filtered through gauze, poured into tubes, centrifuged, a viral suspension is taken and the preparation Viryn KD is prepared. |