BACILLUS TRANSFORMATION, TRANSFORMANTS AND MUTANT LIBRARIES
摘要
The present invention provides methods for building DNA constructs in vitro, transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ.
申请公布号
EP1309677(B1)
申请公布日期
2008.07.09
申请号
EP20010957519
申请日期
2001.08.10
申请人
GENENCOR INTERNATIONAL, INC.
发明人
DIAZ-TORRES, MARIA, R.;SCHELLENBERGER, VOLKER;SELIFONOVA, OLGA, V.;MORRISON, THOMAS, B.;LEE, EDWIN, W.