发明名称 |
AMPLIFICATION METHOD OF METHYLATED OR UNMETHYLATED NUCLEIC ACID |
摘要 |
It is intended to provide a gene amplification method whereby both of a m ethylated nucleic acid and an unmethylated nucleic acid occurring in a biolo gical sample can be amplified and, moreover, the amplification rate(s) of th e methylated nucleic acid and/or unmethylated nucleic acid can be appropriat ely controlled. This object can be achieved by an amplification method which comprises using a nonspecific primer hybridizable with both methylated and unmethylated nucleic acids and a specific primer specifically hybridizable w ith a methylated or unmethylated nucleic acid, and an amplification method w hich further comprises varying the mixing ratio of these primers to thereby vary the amplification rate of a methylated nucleic acid or an unmethylated nucleic acid.
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申请公布号 |
CA2687171(A1) |
申请公布日期 |
2008.12.11 |
申请号 |
CA20082687171 |
申请日期 |
2008.06.03 |
申请人 |
BIO-DIXAM LLC |
发明人 |
TANAKA, NORIAKI;NAGASAKA, TAKESHI;SASAMOTO, HIROMI;MATSUBARA, NAGAHIDE |
分类号 |
C12N15/09;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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