| 发明名称 |
METHOD FOR CULTURING STEM CELL DERIVED FROM ADIPOSE TISSUE |
| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method for separating a stem cell derived from an adipose tissue from a cell cluster obtained by digesting the adipose tissue in high purity and culturing the stem cell. <P>SOLUTION: The method for culturing the stem cell derived from the adipose tissue includes: an extraction step S1 of extracting a cell from a cell suspension obtained by degrading the adipose tissue; a seeding step S2 of seeding the extracted cell into a culture vessel in which the surface treatment is not carried out for improving adhesiveness; and a culturing step S3 of culturing the seeded cell while exchanging a culture medium. The initial exchange of the culture medium in the culturing step S3 is carried out after 16-24 h from the start of the seeding of the cell. <P>COPYRIGHT: (C)2009,JPO&INPIT</p> |
| 申请公布号 |
JP2008295396(A) |
申请公布日期 |
2008.12.11 |
| 申请号 |
JP20070146757 |
申请日期 |
2007.06.01 |
| 申请人 |
OLYMPUS CORP |
发明人 |
MASUDA YOSHIKO;MATSUBARA YOSHINORI |
| 分类号 |
C12N5/07;C12N5/074;C12N5/0775 |
主分类号 |
C12N5/07 |
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