An apparatus for honeybee artificial insemination is provided to allow the artificial insemination to be carried out effectively by the collection and injection of the semen of a honeybee. An apparatus for honeybee artificial insemination comprises a base member(10); a stand(20) which is fixed at the upper surface of the recessed part(10a) of the base member and is provided with a rotating knob(22) combined to a sliding groove(21) formed in the length direction; a supporting rod(60) which is provided with pincettes(61) expanding the genital organs of the queen bee confined in the container(30); and an injector member(80) which is combined to the one side of a position control member(70) to collect the semen of a male bee or to inject the collected semen into a queen bee.
