| 摘要 |
<p>The process for forming a porous medium (MP) for the study of hydrocarbon biodegradation in underground medium such as soil containing hydrocarbons or hydrocarbon reservoir (R), comprises gradually introducing a mixture of solid matrix and bacterial inoculum in a column (CV) to form a homogeneous porous medium depending upon distribution of pore sizes, and completely saturating the porous medium by water and then partially desaturating the water through pores using a unit comprising ceramic microporous membrane (MC) permeable to water and impermeable to air. The process for forming a porous medium (MP) for the study of hydrocarbon biodegradation in underground medium such as soil containing hydrocarbons or hydrocarbon reservoir (R), comprises gradually introducing a mixture of solid matrix and bacterial inoculum in a column (CV) to form a homogeneous porous medium depending upon distribution of pore sizes, completely saturating the porous medium by water and then partially desaturating the water through pores using a unit comprising ceramic microporous membrane (MC) permeable to water and impermeable to air so that the desaturation is homogeneous, refilling the pores by oil and then partially desaturating the oil by drawing oil from oil filled pores using a unit comprising ceramic microporous membrane permeable to oil and impermeable to air to make the desaturation homogeneous. The microporous membranes permeable to water and oil are placed in downstream of a water and oil flow induced by draining of water and oil during partial desaturation of water and oil respectively. The pores are drained by applying a hydrostatic depression using a vacuum pump. The porous medium has a size, a porous network and water and oil saturation corresponding to those of the studied underground medium. Oxygen consumption is measured during biodegradation of the column of porous medium.</p> |
