Methods of using functional 30S subunits

摘要

A method of preparing a peptide in vitro in a defined translation mixture is provided. The method includes providing a defined translation reaction mixture comprising isolated and/or purified 30S subunits comprising small subunit ribosomal proteins and 16S rRNA, 50S subunits comprising large subunit ribosomal proteins, 5S rRNA and 23S rRNA, charged tRNAs for at least one amino acid, an isolated RNA template for translation with at least one codon corresponding to the anticodon in the charged tRNAs, and one or more isolated translation factors.