| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for culturing pancreatic islet cells in vitro for a long period without losing the specific functions. <P>SOLUTION: This method for producing the three-dimensional aggregate comprising the pancreatic islet cells in vitro comprises a process (a) for preparing a honeycomb-like porous body comprising a water-insoluble polymer coated with an extracellular substrate, and a process (b) for culturing the isolated pancreatic islet cells on the honeycomb-like porous body. According to this production method, the three-dimensional aggregate can be formed without forming a single layer, even when the pancreatic islet cells are cultured in vitro. Therefore, the pancreatic islet cell aggregate can be produced and collected without using a protein digestion enzyme such as trypsin, while maintaining the functions of pancreatic islet cells, such as an insulin-secreting ability. Such the pancreatic islet cells are useful for pancreatic islet transplantation treatments. The honeycomb-like porous body holding the three-dimensional aggregate comprising the pancreatic islet cells can be used as bio-artificial pancreas, cell chips or a carrier for transplanting pancreatic islet cells. <P>COPYRIGHT: (C)2009,JPO&INPIT |
