COMPOSITION FOR REPAIRING DEFECT IN SKIN OR GINGIVAL SOFT TISSUE AND METHOD OF CULTURING AUTOLOGOUS FIBROBLASTS

摘要

[PROBLEMS] To provide a method of producing an autologous fibroblast aggregate for producing autologous fibroblasts having been aggregating in a fibrous state, and an aggregate produced by this method. To provide a composition for repairing a defect in a skin or gingival soft tissue which can remain in the area to be treated over a long period of time and thus exhibit a continuous therapeutic effect, and an injection having this composition. [MEANS FOR SOLVING PROBLEMS] The above problems can be solved by a method of producing an autologous fibroblast aggregate which comprises the step of culturing autologous fibroblasts in a culture medium containing vitamin C and the step of culturing the autologous fibroblasts by a procedure selected from among rotary culture, shaken culture and slant culture so as to form a fibrous aggregate due to the extracellular matrix containing collagen that is secreted by the autologous fibroblasts, the aggregate thus produced and so on.