SYSTEM AND METHOD FOR PROTEOMICS

摘要

Significantly higher yield and better resolution in pi gels are obtained by creating traps having two or more layers of gel (26, 28) containing closely stepped immobilized pH buffers. Proteins move from a pH at which they are negatively charged towards an anode at which they are positively charged. The gel layers (26, 28) containing immobilized pH buffers trap the proteins when the immobilized buffer pH and the protein pi are approximately the same. The protein is trapped within the second layer and not on the surface of or interface of the second layer.