发明名称 |
Expression system for enhancing solubility and immunogeneicity of recombinant proteins |
摘要 |
Expression system for enhancing solubility and immunogenicity of recombinant proteins. The expression system includes a protein expression vector that contains a chimeric gene encoding a chimeric protein. The chimeric protein contains three polypeptidyl fragments: (a) a first polypeptidyl fragment at the N-terminal end of the chimeric protein that contains a protein transduction domain (PTD) or a fragment thereof having HIV Tat PTD activity; (b) a second polypeptidyl fragment at the C-terminal end of the first polypeptidyl fragment that contains a J-domain or a fragment thereof having heat shock protein 70 (Hsp70)-interacting activity; and (c) a third polypeptidyl fragment at the C-terminal end of the second polypeptidyl fragment that contains a target protein or polypeptide.
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申请公布号 |
US2008274508(A1) |
申请公布日期 |
2008.11.06 |
申请号 |
US20080149606 |
申请日期 |
2008.05.05 |
申请人 |
STRESSPRO BIOMEDICINE INCORPORATION;ANIMAL TECHNOLOGY INSTITUTE TAIWAN |
发明人 |
CHEN MING-YU;CHUANG CHIN-KAI;SU YU-HSYU;FAN CHIU-TING;YU JUNG-CHUAN;LEE WEN-CHUAN |
分类号 |
C12P21/04;C07K7/00;C07K14/00;C12N1/20;C12N15/00;C12N15/11 |
主分类号 |
C12P21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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