发明名称 |
A RECOMBINANT TYPE II RESTRICTION ENDONUCLEASE, NMEAIII, AND A PROCESS FOR PRODUCING THE SAME |
摘要 |
A protein is described that has an amino acid sequence characterized by at least 90% sequence identity with SEQ ID NO: 24, the protein being capable of recognizing a sequence consisting of 5'-GCCGAG-3' within the double-stranded DNA and cleaving the substrate predominantly at 21/19 nucleotides from the recognition site. A method is also described that utilizes the protein for creating a DNA tag for use as a unique identifier for paired end sequencing of DNA or serial analysis of gene expression.
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申请公布号 |
WO2008083065(A9) |
申请公布日期 |
2008.10.02 |
申请号 |
WO2007US88522 |
申请日期 |
2007.12.21 |
申请人 |
NEW ENGLAND BIOLABS, INC.;MORGAN, RICHARD, D. |
发明人 |
MORGAN, RICHARD, D. |
分类号 |
C12N9/22;C12N15/09;C12N15/55;C12Q1/34;C12Q1/68 |
主分类号 |
C12N9/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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