发明名称 |
Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method |
摘要 |
A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
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申请公布号 |
US7429655(B2) |
申请公布日期 |
2008.09.30 |
申请号 |
US20030683386 |
申请日期 |
2003.10.14 |
申请人 |
KANKYO ENGINEERING CO., LTD.;NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCEAND TECHNOLOGY |
发明人 |
KURANE RYUICHIRO;KANAGAWA TAKAHIRO;KAMAGATA YOICHI;KURATA SHINYA;YAMADA KAZUTAKA;YOKOMAKU TOYOKAZU;KOYAMA OSAMU;FURUSHO KENTA |
分类号 |
C07H21/04;C12P19/34;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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