| 摘要 |
FIELD: chemistry. ^ SUBSTANCE: claimed invention relates to new oligosaccharides modified with fluorophorous marker, namely, 4-O-methylumbelliferyl beta-D-xylopiranosil-(1,4)-bis-[O-beta-D-xylopiranosil-(1,4)]-beta-D-xylopiranoside, 4-O methylumbelliferyl beta-D-xylopiranosil-(1,4)-tris-[O-beta-D-xylopiranosil-(1,4)]-beta-D-xylopiranoside, 4-O-methylumbelliferyl beta-D-xylopiranosil-(1,4)-tetrakis-[O-beta-D-xylopiranosil-(1,4)]-beta-D-xylopiranoside. It is intended for measuring activities of both pure and coarse preparations of beta-xylanases. The invention also relates to fermentative synthesis of oligosaccharides with formula: , which lies in incubation of beta-xylosidase from Aspergillus sp. at 37C in neutral reaction medium in presence of aryl-beta-D-xylopiranoside and 4-O-methylumbelliferyl-beta-D-xylopiranoside. ^ EFFECT: possibility to measure activities of both pure and coarse preparations of beta-xylanases. ^ 4 cl, 1 dwg, 2 tbl |
