CLINICAL SAMPLE PREPARATION ON A MICROFLUIDIC PLATFORM

摘要

Genotyping clinical samples (e.g. whole blood and buccal swabs) for molecular diagnostics is typically performed in laboratories using specialized equipment by trained operators. The present invention provides for an integrated microfluidic approach wherein cell lysis, molecules of interest cleanup, molecules of interest isolation/purification and molecules of interest transport approach is demonstrated. A magnet is focussed within the microfluidic channel and facilitates the magnetic bead movement which binds the molecules of interest. Further a procedure is developed to indefinitely re-use the chips with no notable degradation in performance.