Methods for producing members of specific binding pairs

摘要

PCT No. PCT/GB92/00883 Sec. 371 Date Mar. 31, 1994 Sec. 102(e) Date Mar. 31, 1994 PCT Filed May 15, 1992 PCT Pub. No. WO92/20791 PCT Pub. Date Nov. 26, 1992The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display packages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genetic material of rgdps produced encodes a polypeptide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combinations. In one embodiment of the invention a method employs "chain shuffling" in the production of sbp members of desired specificity for a counterpart sbp member. Selection procedures are also described.