A NOVEL POTENTIOMETRIC CHOLESTEROL SENSOR FOR THE QUANTITATIVE ESTIMATION OF TOTAL CHOLESTEROL IN HUMAN BLOOD SERUM

摘要

The present invention provides a novel sensor for the estimation of total cholesterol in human blood serum using molecular imprint of cholesterol. Human blood serum contains 150-250 mg/dl cholesterol. The present invention provides a sensor device and a process for the fabrication of ISFET (Ion Selective Field Effect Transister) coated with molecular imprint of cholesterol on the SiO₂ + Si₃N₄ dielectric gate of the said electrode. The molecular imprint of cholesterol (MIPC) anchored on to a silica matrix sensing material is identified as a sensing material which has specific selectivity towards the cholesterol in presence of other organic constituents in human blood serum.