Molecules suitable for binding to a metal layer for covalently immobilizing biomolecules

摘要

The present invention provides materials and devices for immobilizing functional organic bio-molecules through a covalent bond via a thiolate or disulfide monolayer to a metal surface wherein an extra activation step of the surface layer or an activation step of the functional bio-molecules or bio-receptors is avoided. The mono-layer can contain two moieties, one having a group that resist non-specific adsorption and another group that directly reacts with functional groups on the bio-molecules. In addition, poly(ethylene oxide) groups are incorporated in the monolayer surfaces in order to resist the non-specific adsorption and to enhance specific affinity interactions. The invention also provides a sensor device for performing reproducible, sensitive, specific and stable bio-analysis.