METHODS FOR CULTURING MESENCHYMAL STEM CELL AND COMPOSITIONS COMPRISING MESENCHYMAL STEM CELL FOR REPAIRING SKIN DEFECTS

摘要

A method for culturing a mesenchymal stem cell is provided to obtain adipose tissue-derived mesenchymal stem cells having higher expression level of type I pro-collagen and lower expression level of pro-MMP-1 efficiently. A pharmaceutical composition is provided to be a cell therapeutic agent to ameliorate or treat successfully skin defects, thereby being completely free from immunological adverse effects. A method for culturing a mesenchymal stem cell highly expressing collagen comprises the steps of: (a) culturing a mesenchymal stem cell isolated from an adipose tissue in a serum-containing medium; and (b) culturing the mesenchymal stem cell in a serum-free medium, wherein the expression level of type I pro-collagen of the mesenchymal stem cell cultured in the step (b) is higher than that of fibroblast and the expression level of pro-MMP-1(pro-matrix metalloprotease-1) of the mesenchymal stem cell cultured in the step (b) is lower than that of fibroblast. A pharmaceutical composition for repairing a skin defect comprises a pharmaceutically effective amount of adipose tissue-derived mesenchymal stem cells and a pharmaceutically acceptable carrier.