APPLICATION OF DNA-DIAGNOSTICS FOR PRESENCE OF POLYMORPHISM R353Q IN GENE OF FACTOR VII OF BLOOD COAGULATION FOR ESTIMATION OF PREDISPOSITION TO DEVELOPMENT OF HABITUAL NONCARRYING OF PREGNANCY AND METHOD OF PREDISPOSITION ESTIMATION TO THIS DISEASE BY MEANS OF ANALYSIS OF COMBINATION OF POLYMORPHISMS C677T OF GENE MTHFR AND OF GENE R353Q OF FACTOR VII

摘要

FIELD: technological processes. ^ SUBSTANCE: invention is related to the field of molecular biology and genetics and may be used in medicine for determination of risk of development of habitual noncarrying of pregnancy (HN). Correlation was established between polymorphism R353Q of gene of blood coagulation factor VII and development of HN, and possibility of using results of DNA analysis for the presence of the mentioned polymorphism is shown in estimation of genetic predisposition to this disease. Method is suggested to predict predisposition for development of HN, which includes simultaneous testing of DNA for presence of polymorphism R353Q of blood coagulation gene factor VII and polymorphism C677T of gene methylene- tetrahydropholatereductase (MTHFR), determination of patient genotype with account of data on two investigated genes and drawing conclusion, where genotype RRTT is qualified as highly unfavorable, genotypes RRCC, RRCC and RQTT - as unfavorable, genotypes RQCC and RQCT - as favourable prognostic criteria. ^ EFFECT: increase of prognostication efficiency. ^ 2 cl, 7 dwg, 1 tbl, 5 ex