Chromatography apparatus having diffusion-bonded and surface-modified components

摘要

A microfluidic device for separating a sample by chromatography includes diffusion bonded metallic sheets joined together to create a hermetically sealed interface between each adjacent metallic sheet without the introduction of a secondary material. Enclosed within the diffusion bonded sheets is a separation channel accessible by at least one of an inlet or an outlet. The separation channel is packed with micrometer-sized particles serving as a stationary phase in a chromatographic separation. Wetted surfaces of the separation channel include a coating of an organic material at least one monolayer thick.

主权项

1. A microfluidic device for separating a sample by chromatography, the microfluidic device comprising:
diffusion bonded metallic sheets, each metallic sheet having a substantially similar composition, the diffusion bonded metallic sheets joined to create a hermetically sealed interface between each adjacent metallic sheet without the introduction of a secondary material and to enclose a separation channel within the diffusion bonded metallic sheets accessible by at least one of an inlet or an outlet to the separation channel; wetted surfaces of the separation channel coated with an organic material at least one monolayer thick, a deposited layer of inorganic-oxide positioned between the wetted surfaces of the separation channel and the organic material, and the coated separation channel packed with micrometer-sized particles serving as a stationary phase in a chromatographic separation.