PRODUCTION OF WEAKLY ACTIVE OR INACTIVE MUTANTS OF ALKALINE PHOSPHATASE AND THEIR EXPRESSION IN YEAST

摘要

The invention concerns a method for the recombinant production or expression of eukaryotic alkaline phosphatase mutants in yeast cells wherein the specifically introduced mutations result in a reduction of the specific AP activity by at least a factor 1:100. The invention also concerns a method for inserting correspondi ng nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns corresponding vectors and host strains.