发明名称 |
MALIC ACID PRODUCTION IN RECOMBINANT YEAST |
摘要 |
We disclose a recombinant yeast, wherein the yeast is pyruvate decarboxylase enzyme (PDC) activity negative (PDC-negative) and is functionally transformed with a coding region encoding a pyruvate carboxylase enzyme (PYC) wherein the PYC is active in the cytosol, a coding region encoding a malate dehydrogenase enzyme (MDH) wherein the MDH is active in the cytosol and is not inactivated in the presence of glucose, and a coding region encoding a malic acid transporter protein (MAE). We also disclose a method of producing malic acid by culturing such a yeast in a medium comprising a carbon source and a carbon dioxide source and isolating malic acid from the medium. |
申请公布号 |
EP1954799(A1) |
申请公布日期 |
2008.08.13 |
申请号 |
EP20060827344 |
申请日期 |
2006.11.01 |
申请人 |
TATE & LYLE INGREDIENTS AMERICAS, INC. |
发明人 |
WINKLER, AARON ADRIAAN;DE HULSTER, ABRAHAM FREDERIK;VAN DIJKEN, JOHANNES PIETER;PRONK, JACOBUS THOMAS |
分类号 |
C12N1/00;C12N1/16;C12P7/46 |
主分类号 |
C12N1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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