Auto-Regulated Expression Of Bacterial Isopentenyltransferase Gene Promotes T-DNA Transformation In Soybean

摘要

Agrobacterium-mediated plant transformation with a transgene of interest is accomplished with high frequency of transformation by using a specially constructed vector. The vector combines the transgene of interest with an autoregulating promoter, such as the P_SAG12 promoter, that controls expression of an IPT coding region. The IPT coding region may be replaced by another ORF coding for the expression of a polypeptide affecting at least one plant cell cycle pathway selected from the group consisting of cytokinin, auxin, and sugar pathways.