TRI-DEMETHYLATION-CAPABLE PROTEIN COMPLEX, METHOD OF ITS PREPARATION AND ITS USE

摘要

The invention relates to a multiple-specificity demethylase complex compr ising a Jumonji C (JMJC) domain-containing enzyme, a process of its preparat ion and its use. It is the object of the present invention to provide enzyme s having histone demethylating activity, particularly having broad-specifity demethylating activity and, more particularly, having tridemethylating acti vity. Furthermore, it is an object of the present invention to provide enzym es having demethylating activity, more particularly having tri-demethylating activity and targeting specific genes, for example the androgen receptor (A R). Moreover, it is an object of the present invention to provide a process of obtaining and isolating such enzymes. A further object of the present inv ention is to demonstrate such enzyme proteins for use in the medical field, particularly in the inhibition of prostate cancer, for screening purposes an d for preventing the expression of AR, progesterone receptor and glucocortyc oid receptor target genes by RNAi mediated knockdown of JMJD2C activity.