发明名称 |
Sperm protein 17 for the diagnosis and treatment of cancer |
摘要 |
The results herein identify Sp17 as a novel cancer-testis antigen in multiple myeloma. Sp17 recombinant protein was generated from E. coli. A CD8 predominant CTL line was generated that was able to lyse autologous targets in a Sp17-dependent HLA class I-restricted manner, using dendritic cells as the antigen-presenting cells and DOTAP to deliver the Sp17 protein to the dendritic cells. A combination of HLA-matched and mismatched antibody-enriched fresh myeloma tumor cells and myeloma cell lines were used as targets for the recombinant protein-propagated CTL. The findings of target cell lysis suggest that the Sp17 protein produced by Sp17<SUP>+</SUP> tumor cells are processed and presented in vivo and that the CTL epitopes are presented in association with HLA class I molecules in a concentration and configuration recognized by recombinant protein-propagated CTL.
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申请公布号 |
US7405077(B2) |
申请公布日期 |
2008.07.29 |
申请号 |
US20020082959 |
申请日期 |
2002.02.26 |
申请人 |
ALBANY MEDICAL COLLEGE |
发明人 |
LIM SEAH H.;CHIRIVA-INTERNATI MAURIZIO;WANG ZHIQING |
分类号 |
C12N5/08;A61K39/00;A61K47/48;C07K14/47;C12P21/08;C12Q1/68;G01N33/574 |
主分类号 |
C12N5/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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