| 摘要 |
A method for screening materials with neuronal regeneration effect using a tissue fragment of the spinal cord is provided to be usefully used to develop a therapeutic agent of neuronal diseases. A method for screening neuronal regeneration materials comprises the steps of: (a) culturing a tissue fragment obtained from the spinal cord; (b) treating the tissue fragment under the culturing with a toxic compound to damage the tissue fragment; (c) treating the damaged tissue fragment with a candidate material expected to have the neuronal regeneration effect; and (d) screening the candidate material showing significant neuronal regeneration effect by comparing the result with a negative control group not-treated with the candidate material, wherein the candidate material is a compound, a growth factor, cytokine, a mesenchymal stem cell derived factor, an extracellular matrix protein, a protein involved with growth of axon, or a factor involved with intracellular signal transduction and the toxic compound is lysolecithin inducing demyelination of the axon of the spinal cord. Further, the damage is demyelination.
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