METHOD AND DEVICE FOR DETECTING THE PRESENCE OF A SINGLE TARGET NUCLEIC ACID IN A SAMPLE

摘要

A method comprises loading a sample portion into a first porous structure, subjecting the sample portion to an amplification step, and determining whether the sample portion contains at least one molecule of a target nucleic acid. If the sample portion contains a single molecule of the target nucleic acid, the sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification. Also, a microfluidic device comprising a porous sample structure and a sample portion positioned in the porous sample structure. Also, a microfluidic device comprising a porous sample structure and an amplification targeting reagent positioned in the porous sample structure.