METHOD OF FORMING AUTOAGGREGATE ON MICROPARTICLE AND METHOD OF DETECTING TARGET ANALYTE

摘要

It is intended to provide a method, in which the sensitivity in detecting a target analyte on a microsphere can be elevated and plural items can be detected at the same time. The presence of at least one target analyte in a sample is detected by bonding a microsphere having a fluorescent substance on the surface and the target analyte to a self assembly substance formed by using two oligonucleotide probes having complementary base sequence regions which are hybridizable with each other via the target analyte, thus forming a self assembly substance-bonded particle, and then analyzing the self assembly substance-bonded particle.