SSR PRIMER DERIVED FROM LAWN GRASS AND USE THEREOF
摘要
<p>An SSR(simple sequence repeat) primer pair is provided to be very useful for DNA profiling of lawn grass by detecting DNA polymorphism of the lawn grass effectively, thereby being used to analyze whether a newly introduced genetic resource is duplicated with a conventional resource and securing novelty of the newly introduced genetic resource and identifying species of the lawn grass. An SSR primer pair comprises two sequences selected from the group consisting of SEQ ID : NOs. 1 to 60 and is derived from lawn grass(Zoysia japonica steud). A method for detecting DNA polymorphism of the lawn grass comprises the steps of: (a) extracting genome DNA from the lawn grass; (b) subjecting the extracted genome DNA as a template to PCR using the SSR primer pair; and (c) isolating PCR products obtained from the step(b) by size. A kit for detecting the DNA polymorphism of the lawn grass or identifying species of the lawn grass comprises the SSR primer pair.</p>
申请公布号
KR100842429(B1)
申请公布日期
2008.07.01
申请号
KR20070035739
申请日期
2007.04.11
申请人
REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION)
发明人
MA, KYUNG HO;PARK, YONG JIN;LEE, GI AN;LEE, JUNG RO;LEE, SOK YOUNG;GWAG, JAE GYUN;KIM, TAE SAN;CHO, EUN GI