| 摘要 |
The invention relates to transgenic non-human mammals carrying two transgenes in the genome thereof, to a method for producing said mammals and the use thereof, and cell tissue, cells and cell constituents thereof. The fields of application of the invention are the pharmaceutical industry and life sciences: biology, biochemistry, biotechnology, medicine, preferably the prevention and treatment of cancer, especially lung cancer. The aim of the invention is to provide an improved animal model which forms different types of tumours that can be compared with human tumours, especially lung tumours, and the metastases thereof. The aim of the invention is also to provide a non-human mammal with such improved characteristics and/or the cell tissue, cells and cell constituents thereof, and a method and means for the production of said mammal, and the use of the same. To this end, a doubly transgenic non-human mammal is provided with a genome containing a) a coding DNA sequence (1) which codes for a proto-oncogene and is operatively connected to at least one regulatory sequence, and b) a coding DNA sequence (2) which codes for an oncogene and is operatively connected to at least one regulatory sequence. The invention also relates to corresponding doubly transgenic tumour cell clones for use in the in vitro analysis of the pathogenesis of lung tumours and of substances or treatment methods for lung carcinomas. |
