| 摘要 |
<p>The present invention concerns a method of producing a desired gene produ ct in a recombinant gene expression system, said method comprising expressin g said gene from a Pm promoter-based expression system using at least two mu tant elements selected from: (i) a mutant Pm promoter; (ii) a mutant mRNA le ader; and (iii) a mutant XyIS; wherein said mutant elements each comprise on e or more mutations which enhance expression of said desired gene. Particula rly combinations of a mutant Pm promoter and a mutant mRNA leader are concer ned. Isolated nucleic acid molecules, vectors, host cells, libraries, expres sion systems, methods of enhancing expression, obtaining nucleic acid molecu les and identifying combination mutants which enhance expression, artificial ly constructed operons and their uses are also encompassed.</p> |
