| 摘要 |
The invention relates to methods for the base sequencing of deoxyribonucleic acid or ribonucleic acid, comprising the following steps: (1) immobilising DNA or RNA single strands on a planar support; (2) focussing a laser beam on a single, immobilised single strand; (3) producing DNA or RNA complimentary strand of said immobilised, focused single strand by adding a solution containing (i) at least one luminescence-tagged nucleotide on the bases of adenine, cytosine, guanine and thymine for producing a DNA or RNA complementary strand or at least one luminescence-tagged nucleotide of the bases adenine, cytosine, guanine and uracil for producing an RNA complementary strand and (ii) a polymerase, each insertion of a luminescence-tagged nucleotide into the complementary strand being detected with a single-molecule detector and the luminescence signal of the previous luminescence-tagged nucleotide being deleted before the next luminescence-tagged nucleotide is inserted. |
