| 摘要 |
This invention relates to methods and devices that improve cell culture e fficiency. They include the use of gas permeable culture compartments that r educe the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration o f gas permeable materials into the traditional multiple shelf format to reso lve the problem of non-uniform culture conditions. They include culture devi ces that use surfaces comprised of gas permeable, plasma charged silicone an d can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of bene fits accrue, including more optimal culture conditions during scale up and m ore efficient use of inventory space, incubator space, and disposal space. F urthermore, labor and contamination risk are reduced. |
