| 摘要 |
The invention relates to a method for the enantioselective enzymatic redu ction of secodione derivatives of general formula (I), wherein the ring stru ctures comprise no, one, or several heteroatom/s, R1 represents hydrogen or a C1-C4alkyl group, R2 represents hydrogen, a C1-C8 alkyl group, or a protec tive OH- group known in prior art, such as an ester, R3 represents hydrogen, a methyl group, or a halide, the structural element (A) represents a benzen e ring or a C6 ring containing 0, 1, or 2 C-C double bonds, positions 6/7 or 7/8 optionally contain a double bond, and the carbon is independently subst ituted with hydrogen, a C1-C4 alkyl group, a halide, or a phenyl group in po sitions 1, 2, 4, 5, 6, 7, 8, 9, 11, 12, and 16. In said method, the secodion e derivative is reduced by means of an oxidoreductase/dehydrogenase in the p resence of NADH or NADPH as a cofactor. According to the invention, the seco dione derivative is used at a concentration of >=10 g/l in the reaction b atch, and the oxidized NAD or NADP cofactor formed by the oxidoreductase/deh ydrogenase is continuously regenerated. |
